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Soft Tissue Protocol 750 ng of DNA is labeled, stained, and homogenized using the DNA Labeling Kit- LS (Bionano; 80005). Stained DNA is loaded onto the Saphyr chip G1.2 and the chip is scanned in order to image the labeled DNA using the Sap.
Bionano sample kits are optimized for isolating and purifying HMW DNA in a process that is gentler than existing HMW DNA extraction methods. The resulting purified DNA, several megabases in length, is optimal for use with Bionano systems.
The Cell Culture DNA Isolation Protocol involves immobilizing a low complexity biological material in an agarose matrix for subsequent Proteinase K digestion followed by RNAse treatment and washes.
Bionano Sample Preparation Kits provide the reagents and protocols needed to extract and label ultra-high molecular weight DNA for use on the Saphyr® system. These kits are optimized for performing optical genome mapping applications on a variety of sample types.
IAB offers a complete workflow for extraction of higher quality DNA and RNA using the Ionic Purification System with purification kits by Bionano. This system utilizes isotachophoresis to increase yields of nucleic acids from FFPE samples and to prevent further damage during the isolation process.
This Bionano Prep SP Tissue and Tumor DNA Isolation Protocol can provide ultra-high molecular weight (UHMW) gDNA in less than 6 hours from a batch of up to 8 samples (up to 4 recommended for novice users) with approximately 10 mg fresh or fresh frozen tissue or tumor.
Bionano Prep Kits™ provide the critical reagents and protocols needed to extract and label high molecular weight (HMW) DNA for use on the Irys ® and Saphyr™ genome mapping systems. Bionano kits are optimized for performing Bionano genome …
Bionano provides a suite of nucleic acid purification sample prep kits for simple and automated isolation of genomic DNA and RNA from a range of valuable sample types. Once the desired nucleic acid has been isolated, you can go straight into the next step of your workflow.
This Bionano Prep SP Blood and Cell Culture DNA Isolation Kit can provide ultra-high molecular weight (UHMW) gDNA in less than 4 hours for EDTA-collected blood and mammalian cell cultures.
This Bionano Prep® SP Fresh Cells DNA Isolation Protocol can provide ultra-high molecular weight (UHMW) gDNA in less than 4 hours from 1.5 million mammalian cells.
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